Arabidopsis nonresponding to oxylipins locus NOXY7 encodes a yeast GCN1 homolog that mediates noncanonical translation regulation and stress adaptation.

Stress adaptation and translational regulation was studied using noxy7 (nonresponding to oxylipins7) from a series of Arabidopsis thaliana mutants. We identified the noxy7 mutation in At1g64790, which encodes a homolog of the yeast translational regulator General Control Nonderepressible1 (GCN1) that activates the GCN2 kinase; GCN2 in turn phosphorylates the α subunit of the translation initiation factor eIF2. This regulatory circuit is conserved in yeast and mammals, in which phosphorylated eIF2α (P-eIF2α) facilitates stress adaptation by inhibiting protein synthesis. In phenotypic and de novo protein synthesis studies with Arabidopsis mutants, we found that NOXY7/GCN1 and GCN2 mediate P-eIF2α formation and adaptation to amino acid deprivation; however, P-eIF2α formation is not linked to general protein synthesis arrest. Additional evidence suggested that NOXY7/GCN1 but not GCN2 regulates adaptation to mitochondrial dysfunction, high boron concentration, and activation of plant immunity to infection by Pseudomonas syringae pv tomato (Pst). In these responses, NOXY7/GCN1 acts with GCN20 to regulate translation in a noncanonical pathway independently of GCN2 and P-eIF2α. These results show the lesser functional relevance of GCN2 and P-eIF2α in plants relative to other eukaryotes and highlight the prominent role of NOXY7/GCN1 and GCN20 in regulation of translation and stress adaptation in plants.

9-Lipoxygenase-Derived Oxylipins Activate Brassinosteroid Signaling to Promote Cell Wall-Based Defense and Limit Pathogen Infection.

The oxylipins, a large family of oxygenated lipid derivatives, regulate plant development and immunity. Two members of the 9-lipoxygenase (9-LOX) oxylipin pathway, 9-hydroxyoctadecatrienoic acid and 9-ketooctadecatrienoic acid, control root development and plant defense. Studies in Arabidopsis (Arabidopsis thaliana) using a series of 9-hydroxyoctadecatrienoic acid- and 9-ketooctadecatrienoic acid-insensitive nonresponding to oxylipins (noxy) mutants showed the importance of the cell wall as a 9-LOX-induced defense component and the participation of NOXY proteins in signaling cell wall damage. Here, we examined 9-LOX signaling using the mutants lox1lox5, which lacks 9-LOX activity, and noxy2-2, which shows oxylipin insensitivity and mitochondrial dysfunction. Mutants in brassinosteroids (BRs), a class of plant hormones necessary for normal plant growth and the control of cell wall integrity, were also analyzed. Several lines of evidence indicated that 9-LOX-derived oxylipins induce BR synthesis and signaling to activate cell wall-based responses such as callose deposition and that constitutive activation of BR signaling in bri1-EMS-suppressor 1-D (bes1-D) plants enhances this response. We found that constitutive BR signaling in bes1-D and brassinolide-resistant 1-1D (bzr1-1D) mutants conferred resistance to Pseudomonas syringae. bes1-D and bzr1-1D showed increased resistance to Golovinomyces cichoracearum, an obligate biotrophic fungus that penetrates the cell wall for successful infection, whereas susceptibility was enhanced in lox1lox5 and noxy2-2. Our results indicate a sequential action of 9-LOX and BR signaling in activating cell wall-based defense, and this response prevents pathogen infection. These results show interaction between the 9-LOX and BR pathways and help to clarify their role in modulating plant defense.

Defense activated by 9-lipoxygenase-derived oxylipins requires specific mitochondrial proteins.

9-Lipoxygenases (9-LOXs) initiate fatty acid oxygenation, resulting in the formation of oxylipins activating plant defense against hemibiotrophic pathogenic bacteria. Previous studies using nonresponding to oxylipins (noxy), a series of Arabidopsis (Arabidopsis thaliana) mutants insensitive to the 9-LOX product 9-hydroxy-10,12,15-octadecatrienoic acid (9-HOT), have demonstrated the importance of cell wall modifications as a component of 9-LOX-induced defense. Here, we show that a majority (71%) of 41 studied noxy mutants have an added insensitivity to isoxaben, an herbicide inhibiting cellulose synthesis and altering the cell wall. The specific mutants noxy2, noxy15, and noxy38, insensitive to both 9-HOT and isoxaben, displayed enhanced susceptibility to Pseudomonas syringae DC3000 as well as reduced activation of salicylic acid-responding genes. Map-based cloning identified the mutation in noxy2 as At5g11630 encoding an uncharacterized mitochondrial protein, designated NOXY2. Moreover, noxy15 and noxy38 were mapped at the DYNAMIN RELATED PROTEIN3A and FRIENDLY MITOCHONDRIA loci, respectively. Fluorescence microscopy and molecular analyses revealed that the three noxy mutants characterized exhibit mitochondrial dysfunction and that 9-HOT added to wild-type Arabidopsis causes mitochondrial aggregation and loss of mitochondrial membrane potential. The results suggest that the defensive responses and cell wall modifications caused by 9-HOT are under mitochondrial retrograde control and that mitochondria play a fundamental role in innate immunity signaling.

Promoter activities of genes encoding ß-galactosidases from Arabidopsis a1 subfamily.

Promoter regions of each of the six AtBGAL gene of the subfamily a1 of Arabidopsis thaliana were used to drive the expression of the ß-glucuronidase gene. The pattern of promoters (pAtBGAL) activity was followed by histological staining during plant development. pAtBGAL1, pAtBGAL3 and pAtBGAL4 showed a similar activity pattern, being stronger in cells and organs in expansion, and the staining decreasing when cell expansion decreased with age. That indicates a consistent involvement of the encoded ß-galactosidases in cells undergoing cell wall extension or remodelling in cotyledons, leaves and flower buds. These promoters were also active in the calyptra cells and in pollen grains. pAtBGAL2 activity showed a clear relationship with hypocotyl elongation in both light and dark conditions and, like pAtBGAL1, pAtBGAL3 and pAtBGAL4, it was detected during the expansion of cotyledons, rosette leaves and cauline leaves. Its activity was also intense in the early stages of flower and fruit development. pAtBGAL5 was the only one among those from the subfamily a1 that was active in the trichomes that appear throughout the plant, indicating a high specificity of the AtBGAL5 protein and its involvement in the cell wall changes that accompany the formation of the trichome. The activity of pAtBGAL5 was also high in radicles and roots, except in the meristematic area of these organs, and during seed formation. Finally, the activity of pAtBGAL12 was mainly detected in meristematic zones of the plant: the leaf primordium, emerging secondary roots and developing seeds, which indicates an involvement in the differentiation process.

Case report: melioidosis imported from West Africa to Europe.

We report the first case of imported melioidosis in Spain from a diabetic immigrant who visited West Africa during the rainy season. Because of the unusual presentation of this disease in Africa, clinical and microbiological diagnosis of imported melioidosis from this continent can be very elusive.

Prolapso rectal completo en el contexto de las enfermedades del suelo pélvico. Nuestra experiencia en la resección por la vía perineal / Complete rectal prolapse in the context of pelvic floor disorders. Our experience in perineal resection

Introducción. El objetivo de este estudio es analizar los resultados obtenidos con la rectosigmoidectomía perineal con levatorplastia anterior, según la técnica de Altemeier, en un grupo determinado de pacientes de edad avanzada y en los que el riesgo quirúrgico y anestésico era muy elevado. De igual forma, se estudia el porcentaje de enfermedades del suelo pélvico asociadas a estos pacientes. Pacientes y método. En el período comprendido entre 1983 y 2002 se estudió retrospectivamente a 68 pacientes, de los que 45 fueron tratados mediante resección perineal por reunir las condiciones de edad avanzada y alto riesgo quirúrgico. Se analizan los resultados, así como las enfermedades perineales asociadas. Se estudian las complicaciones inmediatas y tardías, y la morbimortalidad en dicho grupo de pacientes. Resultados. Durante un seguimiento promedio de 6 años no se ha registrado mortalidad en el grupo total de pacientes y la morbilidad ha sido escasa y de poca relevancia clínica. Sólo se ha observado recidiva en 2 casos (4,4 por ciento) y el tratamiento realizado fue una nueva resección perineal. La frecuencia de enfermedades del suelo pélvico asociadas ha sido del 88,8 por ciento. Conclusiones. El prolapso de recto se asocia frecuentemente con enfermedades multiorgánicas del suelo pélvico. La resección perineal de Altemeier ofrece buenos resultados y permite intervenir a este grupo de pacientes con una mortalidad nula y una morbilidad poco relevante, así como también tratar otras enfermedades del suelo pélvico asociadas (AU)